Changes of Ski expression levels in rat activated astrocytes
10.3969/j.issn.1000-4718.2017.06.002
- VernacularTitle:Ski在大鼠活化星形胶质细胞中表达的变化
- Author:
Xin ZHAO
;
Jiangli KOU
;
Yongqiang GUO
;
Yanchuan PU
;
Kaisheng ZHOU
;
Wei NAN
;
Jing WANG
;
Yamin WU
;
Haihong ZHANG
- Keywords:
Ski;
Glial fibrillary acidic protein;
Astrocytes;
Glial scar
- From:
Chinese Journal of Pathophysiology
2017;33(6):968-974
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the time-dependent change of Ski protein expression in normal and activated astrocytes in rats.METHODS:The astrocytes were obtained from rat cerebral cortex and cultured in vitro.The astrocytes were treated with LPS and scratch injury for activation.Western blot analysis was used to determine glial fibrillary acidic protein (GFAP) and Ski protein levels in activated astrocytes at a series of time points.The indirect immunofluorescence staining method was performed to detect the location of Ski protein in the astrocytes.RESULTS:The protein of GFAP was naturally expressed in the astrocytes, beginning to increase after treated with LPS and scratch injury.Little protein expression of Ski in the normal astrocytes was observed.The Ski protein expression began to increase after treated with 1 mg/L LPS, peaked at 4 d (P<0.05) and then deceased, but was stills higher than that in the normal cells.The protein expression level of Ski after scratch injury was highly consistent with above mentioned.Ski was mainly observed in the nucleus of the normal cells and the cells treated with LPS for 6 d, while it was observed in the cytoplasm 2 and 4 d after treated with LPS.CONCLUSION:The protein of Ski is expressed in the astrocytes, and the expression level is increased in activated astrocytes,mainly located in the nucelus.Ski may plays an essential roles in the processes of activation and proliferation of astrocytes.
