Effects of H2 on osteoclast differentiation of RAW264.7 cells induced by RANKL and RANKL/TNF-α

VernacularTitle:分子氢对RANKL和RANKL/TNF-α诱导的RAW264.7细胞向破骨细胞分化的影响
Author: Jiandong YUAN ; Ju YE ; Congcong WU ; Yangbo LIU ; Xiaojing HUANG
Keywords: H2; RAW264.7 cell; oxidative stress; osteoporosis
From: Chinese Journal of Biochemical Pharmaceutics 2017;37(6):20-22,26
CountryChina
Language:Chinese
Abstract: Objective To discuss the effect of H2 on osteoclast differentiation of RAW264.7 cells induced by RANKL and RANKL/TNF-α.MethodsRAW264.7 cells were treated with H2 in the presence of RANKL and RANKL/TNF-α.RAW264.7 cells viability was assessed by CCK-8.Test the Oxidative Stress of the induced RAW264.7.The number of TRAP-positive cells were counted under light microscopy.The levels of cathepsin K (CTK) and matrix metalloprotein-9(MMP-9) mRNA were analyzed by real-time PCR.ResultsH2 can not influence the RAW264.7 cell viability but can lower oxidative stress.The significant difference(P<0.05) indicated that H2 could significantly decrease the number of TRAP-positive MNCs.The significant difference among the 4 groups in CTK and MMP-9 genes (P<0.05) indicated that H2 could down-regulate their mRNA expression.ConclusionH2 can reduce the oxidative stress and inhibit differentiation of RAW264.7 cells into osteoclasts.