Effects of LPS-mediated IL-6 production on EZH2 expression in ovarian cancer cells and its mechanism
10.3969/j.issn.1005-1678.2017.06.006
- VernacularTitle:卵巢癌细胞中LPS介导的IL-6产生对EZH2表达的影响及机制研究
- Author:
Weiwei SHEN
;
Jie CHEN
;
Shiping LAI
;
Huifen SHAO
- Keywords:
ovarian cancer cell;
lipopolysaccharide;
interleukin-6;
EZH2
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(6):23-26
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to investigate the expression of IL-6 induced by LPS in ovarian cancer and the effect of IL-6 on the expression of EZH2 mRNA and protein in ovarian cancer cell lines.MethodsCulture ovarian cancer cell lines, according to the different medium composition, establish the experimental group and the control group, detect the concentration of IL-6 in the supernatant of ovarian cancer cells by ELISA and the OD values of each group by MTT assay.Western blotting was used to detect the expression of EZH2 protein in ovarian cancer cells.The mRNA expression of EZH2 was detected by RT-PCR.ResultsThe expression of IL-6 in the supernatant of ovarian cancer cells was significantly higher than that in the control group (P<0.05).The proliferation of ovarian cancer cells was inhibited and the difference was statistically significant (P<0.05).The expression of EZH2 protein in ovarian cancer cells was significantly decreased, and there was statistical difference (P<0.05).The mRNA expression of EZH2 was significantly decreased, and there was statistical difference (P<0.05).ConclusionLPS could induce the expression of IL-6 and inhibit cell proliferation in ovarian cancer cells.IL-6 could inhibit the expression of EZH2.
