Preliminary study on protein A in some species of Staphylococcus aureus isolated in Vietnam
- Author:
Do Ngo Lien
- Publication Type:Journal Article
- Keywords:
protein A, Staphylococcus aureus, isolation
- MeSH:
Staphylococcal Protein A;
biosynthesis;
Enzyme-Linked Immunosorbent Assay
- From:Pharmaceutical Journal
2003;322(2):18-21
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
the ability to biosynthesis of protein A from some Staphylococcus aureus strains isolated from the patients of Bach Mai Hospital was studied in the years 2001-2002. The results showed that there were 3 strains (25, 28 and 55) have the ability to synthesize protein A in the highest levels among 8 strains investigated. Using ELISA technique with protein A served as an antigen from Staphylococcus strain 25 to analyse IgG from sera of 32 healthy subjects has determined that the average IgG level from human serum is 14.37mg/ml. This result was supported by the same technique using commercial foreign bio-kit. It is proven that protein A preparation can be used as an antigen in bio-kit capturing specifically IgG from human serum in clinical diagnosis and in immuno-pathological research using ELISA technique
