Establishment of rabbit model for immunogenicity evaluation of pneumococcal conjugate vaccine
10.3760/cma.j.issn.0254-5101.2017.05.010
- VernacularTitle:评价肺炎球菌结合疫苗免疫原性的兔模型的建立
- Author:
Yonghong LEI
;
Xiaoxue ZHANG
;
Chenbao XUE
;
Yulong ZHANG
;
Xinli WANG
;
Kai XU
;
Zheng XIANG
;
Qiang GAO
;
Jisheng LIN
- Keywords:
Rabbit model;
Enzyme-linked immunosorbent assay (ELISA);
Opsonophagocytosis assay (OPA);
Immunogenicity;
13-valent pneumococcal conjugate vaccine (PCV13)
- From:
Chinese Journal of Microbiology and Immunology
2017;37(5):386-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an animal model for evaluating immunogenicity of pneumococcal conjugate vaccine.Methods New Zealand rabbits were intramuscularly administrated with three doses of 13-valent pneumococcal conjugate vaccine (PCV13) with two weeks interval between each injection.Serum samples were collected at different time points before and after vaccination.Quantitative enzyme-linked immunosorbent assay (ELISA) and opsonophagocytosis assay (OPA) that were in conformity with the World Health Organization (WHO) standards were used to detect the concentrations of serotype-specific antibodies and their bactericidal activities.Results The concentrations (Geometric mean concentration, GMC) of serotype-specific antibodies in rabbit serum samples were well correlated with their bactericidal activities (Geometric mean titer, GMT) following vaccination.Moreover, the dynamic changes of GMC and GMT of the same serotype-specific antibody remained consistent as time went by.Conclusion Rabbit model can be used to analyze the immunogenicity of PCV13 vaccine with quantitative ELISA and OPA, which indicates that it is a suitable animal model for evaluating immunogenicity of pneumococcal conjugate vaccine.
