Protective Effect of Different Extraction of Agaricus blazei Murrill on HaCaT Cells Damaged by UVB
10.16466/j.issn1005-5509.2017.06.003
- VernacularTitle:姬松茸不同提取物对UVB致HaCaT细胞光损伤的保护作用研究
- Author:
Yan ZHU
;
Zhen HUANG
;
Xiaoming ZHONG
- Keywords:
Agaricus blazei Murrill;
water extraction;
aqueous extraction;
crude polysaccharide;
HaCaT;
UVB;
protective effect
- From:
Journal of Zhejiang Chinese Medical University
2017;41(6):458-463
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To investigate the protective effects of different extraction of Agaricus blazei Murrill on HaCaT cells damaged by UVB. [Methods] The cell viability which was detected by MTT method was used to determine the impact of different dose of UVB and different extraction of Agaricus blazei Murrill on normal HaCaT cells and HaCaT cells damaged by UVB. [Results] ①The HaCaT cells viability declined after irradiation with UVB, and it was a dose-dependent manner. The cell viability was 53.23%when the dose of UVB was 20mj/cm2. ②The water extraction at the concentration of 0.2~4mg·mL-1, aqueous extraction at the concentration of 0.1~2mg·mL-1 and crude polysaccharide at the concentration of 0.2~1mg·mL-1 could promote the proliferation of HaCaT cells, while aqueous extraction and crude polysaccharide inhibited the proliferation of HaCaT cells at higher concentrations.③The water extraction at the concentration of 1~4mg·mL-1, aqueous extraction at the concentration of 1~2mg·mL-1 and crude polysaccharide at the concentration of 0.5~1mg·mL-1 could enhance the cells viability which was irradiated by 20mj/cm2 UVB (P<0.01). [Conclusion] The different extraction of Agaricus blazei Murrill all could relieve the photo-damage caused by UVB, and it has the potential of anti-photoaging.
