The effect of medium chain triglyceride on blood lipid and expression of leptin in myocardium of rats fed with high fat diet
10.3969/j.issn.1008-9691.2017.03.002
- VernacularTitle:中链三酰甘油对高脂饮食大鼠血脂及心肌瘦素表达的影响
- Author:
Xiaojin YANG
- Keywords:
Medium-chain triglyceride;
High fat diet;
Leptin;
Myocardium
- From:
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
2017;24(3):230-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of medium-chain triglyceride (MCT) on levels of blood lipids and leptin in myocardium tissue.Methods Thirty-two male Sprague-Dawley (SD) rats aged 4 weeks were divided into control group [n = 8, given normal diet prepared by American Nutrition Society (AIN93)], common high fat diet group [HFD group (n = 16), given 23% lard+ 5% corn oil], MCT group (n = 8, given 23% MCT+ 5% corn oil) by random number table, after 8 weeks of feeding, the HFD group was further randomly subdivided into HFD group fed with diet as before and (HFD+ MCT) group whose diet was replaced bythe diet in MCT group, each subgroup 8 rats, and all the rats continued to be fed for further 8 weeks. The rats' diet intake and the changes of their body weight were observed weekly. After total 16 weeks, the rat tail venous blood was collected, the levels of triglycerides (TG), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) in serum were measured; The expression of leptin mRNA in rat myocardium was measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the contents of leptin in myocardium tissue and serum were measured by enzyme-linked immunosorbent assay (ELISA).Results Compared with the control group, the difference in daily food intake quantity of any one of the high fat diet groups was not statistically significant (allP > 0.05); the final body weight, blood lipid level , the level of leptin mRNA expression in myocardium, contents of leptin in myocardium and serum in the HFD, MCT, and HFD+ MCT groups were all significantly higher, the increase was the most pronounced in the HFD group [body weight (g): 395±13 vs. 326±10, TG (mmol/L): 2.01±0.06 vs. 1.46±0.07, TC (mmol/L): 2.67±0.12 vs. 1.62±0.06, LDL-C (mmol/L): 1.72±0.12 vs. 1.10±0.07, HDL-C (mmol/L): 2.07±0.03 vs. 1.56±0.05, mRNA espression (gray value): 0.357±0.023 vs. 0.202±0.004, leptin in myocardium (pg/mg): 19.30±2.81 vs. 11.50±1.40, leptin in serum (μg/L): 14.63±4.50 vs. 8.21±2.98, allP < 0.05], and the least increase was in the MCT group [respectively was (363±9) g, (1.65±0.07) mmol/L, (2.30±0.06) mmol/L, (1.38±0.05) mmol/L, (1.85±0.04) mmol/L, 0.255±0.005 (gray value), (14.44±1.87) pg/mg, (10.56±2.20)μg/L].Conclusion The high fat diet can significantly increase the body weight, blood lipid level and the contents of leptin in rat myocardium and serum, and MCT can alleviate the above effects of high fat diet to a certain degree, thus it has the potential of cardiovascular protection.
