Effects of Different Processes on Contents of Three Alkaloid Ingredients in Coridalis Rhizoma
10.3969/j.issn.1005-5304.2017.07.021
- VernacularTitle:不同加工方法对延胡索3种生物碱含量的影响
- Author:
Quanjiang LONG
;
Ying ZHANG
;
Xueqin XU
- Keywords:
Coridalis Rhizoma;
steaming process;
boiling process;
alkaloids;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(7):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of different processes on the contents of three alkaloid ingredients in Coridalis Rhizoma.Methods The contents of three alkaloid ingredients (protopine, dehydrocorydaline, tetrahydropalmatine) were determined by HPLC. Agilent HC-C18(2) column (4.6 mm × 250 mm, 5μm) was set at 35℃; acetonitrile-0.1% phosphoric acid solution (triethylamine adjusted pH 5.3) (26:74) was set as mobile phase; the flow rate was 1.0 mL/min and detection wavelength was set at 280 nm; the injection volume was 10μL. The three components were separated well under these chromatographic conditions. The linear relationship between the mass of each component and the peak area was good (r2≥0.9996). The average recoveries were 100.31%, 99.34%, and 99.34%, respectively (RSD≤2.70%).Results The contents of protopine, dehydrocorydaline, tetrahydropalmatine and the total contents of three alkaloids were higher than other groups in large size samples (diameter of fresh products was 2.0–3.0 cm) which were boiled 5–6 minutes, the medium size samples (diameter of fresh products was 1.5–1.9 cm) which were boiled 2–4 minutes and the small size samples (diameter of fresh products was 0.7–1.4 cm) which were boiled 1–2 minutes. The contents of alkaloid in boiled process were lower than steamed process.Conclusion The Results of study provide an experimental basis for origin processing of Coridalis Rhizoma.
