Extraction Technology Optimization for Organic Acids in Crataegi Fructus by Central Composite De-sign-response Surface Method
10.6039/j.issn.1001-0408.2017.16.22
- VernacularTitle:星点设计-响应面法优化山楂有机酸的提取工艺
- Author:
Keyuan XIONG
;
Danna WAN
;
Feng SHAO
;
Haiping ZHAO
;
Ronghua LIU
;
Huilian HUANG
;
Ming YANG
- Keywords:
Crataegi fructus;
Organic acids;
Extraction technology;
Central composite design-response surface method
- From:
China Pharmacy
2017;28(16):2241-2243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the extraction technology for organic acids in Crataegi fructus. METHODS:Based on sin-gle factor test,using liquid-solid ratio,extraction time and ethanol volume fraction as independent variables,extraction yield of or-ganic acids as dependent variable,central composite design-response surface method was used to optimize the extraction technology of organic acids in Crataegi fructus. RESULTS:The optimal extraction technology was as follow as liquid-solid ratio of 18.5:1, adding 75% ethanol,reflux extraction twice,2.0 h each time. Average extraction yield of organic acids in verification test was 5.22%(RSD=2.70%,n=3),with 1.75% relative error of the predicted value(5.13%). CONCLUSIONS:Optimized extraction technology for organic acids in Crataegi fructus is simple,with good reproducibility and predictability.
