Effects of lack of mitochondria topoisomerase on migration ability of gastric cancer cells
10.3760/cma.j.issn.1008-6706.2017.14.004
- VernacularTitle:线粒体拓扑异构酶缺失对胃癌细胞迁移能力的影响
- Author:
Dandan ZHUANG
;
Jianwei XI
- Keywords:
DNA topoisomerases;
Stomach neoplasms;
Neoplasm metastasis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2017;24(14):2092-2094
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of mitochondrial topoisomerase(TOP1MT) on migration of gastric cancer cells.Methods Through siRNA of TOP1MT,the expression of TOP1MT in MKN45 and MKN28 gastric cancer (GC) cells reduced.The effect on GC cell migration was proved by using scratch test and Transwell experiments.Results Compared with the control group,TOP1MT knockout significantly increased the migration of GC cells of MKN28 and MKN45 (t=48.69,P<0.05;t=23.42,P<0.05).Immunohistochemical staining further proved that lack of TOP1MT was associated with lymph node metastasis in gastric cancer (F=14.97,P<0.05).Conclusion Low expression of TOP1MT can significantly enhance the migration of GC cells.
