Effect of Electroacupuncture on Hippocampal Tissue and Interstitial Fluid Soluble Aβ1-42 Levels in 5-month-old APP/PS1 Transgenic Mice
10.13460/j.issn.1005-0957.2017.06.0744
- VernacularTitle:电针对5月龄APP/PS1转基因小鼠海马组织及组织间液可溶性Aβ1-42水平的影响
- Author:
Qingyun BU
;
Weiguo XUE
- Keywords:
Alzheimer's disease;
Electroacupuncture;
Early intervention;
Brain interstitial fluid;
Microdialysis;
Mice
- From:
Shanghai Journal of Acupuncture and Moxibustion
2017;36(6):744-750
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intervening effect of electroacupuncture on brain interstitial fluid (ISF) and hippocampal soluble Aβ1-42 levels in mice with early Alzheimer's disease.Method Sixteen 5-month-old male APP/PS1 positive transgenic mice were randomized to model and acupuncture groups, 8 mice each in one cage. Isosexual negative transgenic mice constituted a normal control group. Electroacupuncture at Baihui(GV20) and Yongquan(KI1) or binding under the same condition was used as an intervention measure. Mouse hippocampal interstitial fluid was obtained by brain microdialysis. Hippocampal tissue and ISFβ amyloid protein 1-42 levels were measured by ELISA in the three groups of mice at 6, 7, 8 and 9 hrs after intervention to observe the changes. Result Hippocampal tissue and ISF soluble Aβ1-42 levels were significantly higher in the model group than in the normal group (P<0.01). ISF soluble Aβ1-42 levels were significantly lower in the acupuncture group of mice than in the model group (P<0.05) and there were no statistically significant differences in hippocampal Aβ1-42 levels between the two groups (P>0.05). The results of sample detection did not show a tendency to change over time (P>0.05). Conclusion Electroacupuncture can decrease hippocampal soluble Aβ1-42 levels in a mouse model of AD.
