Comparison of the Differences Between External Standard Method and Relative Correction Factor Method for Determination of the Flavonoids from Sorbus Tianschanica Rupr
10.3870/j.issn.1004-0781.2017.07.017
- VernacularTitle:外标法与相对校正因子法测定天山花楸黄酮含量的比较
- Author:
Rong FENG
;
Xiaoping SI
;
Hui TANG
;
Huifang LI
;
Xinyu LIU
- Keywords:
Sorbus tianschanica Rupr.;
Hyperoside;
Rutin;
External standard method;
Correction factor;
relative
- From:
Herald of Medicine
2017;36(7):790-793
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differences between external standard method and relative correction factor method for determination of the flavonoids from Sorbus tianschanica Rupr.Methods Using HPLC external standard method for determination of hyperoside,rutin,isoquercitrin,quercetin-3-O-(6″-O-malonyl)-β-D-glucoside,astragalin and Kaempferol-3-O-(6″-O-malonyl)-β-D-glucopyranoside in Sorbus tianschanica Rupr.,HPLC relative correction factor method was adopted to establish relative correction factor of the other five flavonoids above with hyperoside as reference.The difference was evaluated by comparing the external standard method with the relative correction factor method.Results There was no significant difference between the T test external standard method and relative correction factor method(P>0.05).Conclusion External standard method and relative correction factor method can be used for determination of the flavonoids from Sorbus tianschanica Rupr.,but in the case of lack of reference substance or mass detection,using the relative correction factor method for determination of rutin,hyperoside isoquercitrin,quercetin-3-O-(6″-O-malonyl)-β-D-glucoside,astragalin and kaempferol-3-O-(6″-O-malonyl)-β-D-glucopyranoside in Sorbus tianschanica Rupr.It was more feasible and it can be used as a new quality evaluation method in determination of flavonoid components from Sorbus tianschanica Rupr.
