Effects of mangiferin on tissue factor expressionin vascular endothelial cells and the underlying mechanisms
10.3969/j.issn.1001-1978.2017.07.015
- VernacularTitle:芒果苷对血管内皮细胞中组织因子的影响及其机制
- Author:
Yangyang WANG
;
Huiling YU
;
Yan CHEN
;
Ying CHANG
;
Yaning WEI
;
Yingjian HOU
- Keywords:
mangiferin;
tissue factor;
HUVECs;
PPARγ;
thrombosis;
GW9662
- From:
Chinese Pharmacological Bulletin
2017;33(7):961-965
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of mangiferin on tissue factor(TF) expression in human umbilical vein endothelial cells(HUVECs) and the underlying mechanisms.Methods HUVECs were isolated and primarily cultured in vitro.After the treatment with mangiferin and oxidized low density lipoprotein(oxLDL), TF expression was determined in HUVECs with real-time PCR and Western blot.Results oxLDLinduced the mRNA and protein expression and pro-thrombotic activity of TF in HUVECs.However, the inductive effects of oxLDL were blocked significantly by mangiferin.Furthermore, mangiferin modified TF expression and activity in a dose-dependent manner.Mangiferin was demonstrated to enhance the activity of peroxisome proliferator-activated receptor gamma(PPARγ).In contrast, GW9662, an antagonist of PPARγ, reversed at least partially the suppressive effects of mangiferin on TF.Conclusion Through activating PPARγ, mangiferin suppresses the expression of TF serving pro-thrombotic functions in endothelial cells.
