Effect of salvianolic acid B and tanshinone Ⅱ A on theproliferation of rat thoracic aorta adventitial fibroblast
10.3969/j.issn.1001-1978.2017.07.021
- VernacularTitle:丹酚酸B和丹参酮ⅡA对大鼠胸主动脉外膜成纤维细胞增殖的影响
- Author:
Pei LIU
;
Junqiu FU
;
Tiemei SHAO
;
Zhan JIAO
;
Xue LI
;
Tao WU
;
Shengjun AN
- Keywords:
salvianolic acid B;
tanshinone ⅡA;
salvia miltiorrhiza;
proliferation;
flow cytometry;
cell cycle
- From:
Chinese Pharmacological Bulletin
2017;33(7):991-996
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of salvianolic acid B(SAB) and tanshinone ⅡA(TA) alone or the compatibility of these two effective components on the proliferation of rat vascular adventitial fibroblasts, and to observe the effects of the compatibility of the two on cell proliferation with the stimuation of angiotensin Ⅱ(Ang Ⅱ).Methods The effects of SAB and TA alone or the compatibility of the two on cell proliferation were detected by methyl thiazolyl tetrazolium(MTT) method, and flow cytometry was adopted to detect cell cycle with or without the induction of Ang Ⅱ.Results It was shown that SAB and TA alone could inhibit fibroblast proliferation in different degree.Through a series of concentration screening, three effective concentrations were obtained respectively and then the inhibition of cell growth was detected by Pairwise compatibilities.The results showed that the compatibility of TA(10-8 mol·L-1) and SAB(10-5 mol·L-1) had the most significant inhibitory effect(P<0.01), and they could inhibit cell proliferation, further flow cytometry was adopted to detect drug effects on cell cycle, the results indicated that the compatibility of SAB and TA mainly blocked the cells in G0/G1 phase.Induced by Ang Ⅱ, the compatibility of SAB and TA group, compared with Ang Ⅱ group, blocked thee cells in G0/G1 phase;and compared with combination of SAB and TA group, it mainly blocked cell cycle in S phase.Conclusion SAB and TA have certain inhibitory effect on fibroblast proliferation, also they could inhibit the proliferation induced by Ang Ⅱ, mainly by blocking the cells in G0/G1 phase.
