The effect of PKCζinhibitor on the proliferation and invasion of skin squamous carcinoma cell line A-431
10.11958/20170369
- VernacularTitle:蛋白激酶Cζ抑制剂对皮肤鳞癌细胞A-431增殖和侵袭的影响
- Author:
Yanling XING
;
Jing WU
- Keywords:
protein kinase C;
protein kinase inhibitors;
skin neoplasms;
cell proliferation;
neoplasm invasiveness;
neoplasm metastasis;
A-431
- From:
Tianjin Medical Journal
2017;45(6):566-570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of protein kinase C (PKC)ζinhibitor T5450996 on the proliferation and invasion of skin squamous carcinoma cell line A-431. Methods PKCζinhibitor T5450996 was screened through Z′-LYTE?kit. Cell proliferation assay and cell cycle analysis were used to observe the effects of T5450996 on the proliferation of skin squamous carcinoma A-431 cells. Scratch assay and invasion assay were used to explore the effects of T5450996 on the migration and invasion of skin squamous carcinoma A-431 cells. Results The PKCζinhibitor T5450996 can inhibit the activity of PKCζkinase, and the IC50 value of T5450996 was about 35μmol/L. Compared to the control group, 35μmol/L and 70 μmol/L concentrations of T5450996 significantly suppressed the proliferation of A-431 cells and blocked the cell cycle of A-431 cells. The results of scratch assay and invasion assay indicated that the migration and invasion capacities of A-431 cells were markedly impaired after the treatments with 35μmol/L and 70μmol/L concentrations of T5450996 (P<0.05). However, 20μmol/L concentration of T5450996 showed no significant effect (P>0.05). Conclusion PKCζinhibitor T5450996 significantly inhibits the proliferation and invasion capacities of skin squamous carcinoma cell line A-431, and which may be a small molecular inhibitor with potential applications in the future.
