Protective effects of 3,3′-diindolylmethane on radiation damage of hematopoietic system in mice
10.11958/20170457
- VernacularTitle:3,3′-二吲哚甲烷对小鼠造血系统辐射损伤的保护作用
- Author:
Jiali DONG
;
Lu LU
;
Saijun FAN
- Keywords:
radiation injuries;
hematopoietic system;
reactive oxygen species;
bone marrow cells;
mice;
inbred C57BL;
3;
3′-diindolylmethane
- From:
Tianjin Medical Journal
2017;45(6):580-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of 3,3′-diindolylmethane (DIM) on radiation-induced injury in mouse hematopoietic system. Methods Thirty C57BL/6 mice were randomly divided into control group, 2 Gy irradiation group and 2 Gy irradiation+DIM group (n=10 for each group). Mice of control group received sham irradiation, and the other two groups accepted 2 Gy 137Cs γ-ray total body irradiation. Mice in 2 Gy irradiation +DIM group were intraperitoneally injected 75 mg/kg DIM 30 min before irradiation. Mice of other two groups were treated with reference solution. After 7 d and 15 d of 2 Gy irradiation, the peripheral blood samples were collected to count the number of bone marrow nuclear cells (BMNCs). The level of reactive oxygen species(ROS) was measured by DCFH-DA. The levels of colony forming units-granulocyte-macrophage (CFU-GM) were also detected. Results The numbers of white blood cell (WBC), platelet count (PLT), BMNCs and CFU-GM were significantly decreased and the ROS level of bone marrow cells increased significantly in the irradiated group than those of control group (P<0.05). Compared to 2 Gy irradiation group, the numbers of WBC, PLT, BMNCs and CFU-GM were significantly increased in 2 Gy irradiation +DIM group, and the level of ROS was decreased significantly (P<0.05). Conclusion DIM has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with the decreased ROS level.
