Pathogenic analysis of rat infected hantavirus in Henan province, 2014-2016
10.11958/20170418
- VernacularTitle:2014—2016年河南省鼠类携带汉坦病毒的病原学分析
- Author:
Yanhua DU
;
Yi LI
;
Hong MA
;
Haifeng WANG
;
Bianli XU
;
Xueyong HUANG
- Keywords:
hemorrhagic fever with renal syndrome;
Hantann virus;
genotype;
Henan;
etiology;
phylogenetic analysis
- From:
Tianjin Medical Journal
2017;45(6):648-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the genotyping of hantavirus and investigate the pathogenic features of local rats in Henan province. Methods A total of 600 rats captured in Queshan county, Zhumadian city from 2014-2016 were chosen to find out the major species and density. Rat lung specimens were detected by RT-PCR using partial M and S segment primers, then sequencing and phylogenetic analysis based on M segment (2003-2302 nt) were performed to analyze gene subtype and evolution. Results In the field of Queshan county, major species were sewer rats and apodemus agrarius, and the average density of rats was 1.33%-1.83%. Sewer rats, mus musculus and apodemus agrarius were major species in the residential area, and the average density of rats was 1.36%-1.97%. Hantaviruses were detected by RT-PCR in three captured rats in 2014, and the species were mus musculus, cricetulus triton and sewer rats. Nucleotide homology similarity based M and S segment of three positive products was 100%. Phylogenetic analysis indicated the virus was belonged to S4 subgenotype of Seoul virus, which was similar with the strains in Korea and Hubei province, China. Conclusion The virus from rats in Queshan county, Henan province is seoul virus, S4 subgenotype. It is necessary to take the relevant prevention and control measures to prevent hemorrhagic fever of renal syndrome because of wide host range.
