Preparation and Identification of Main Ingredients in Effective Parts of Xinjiang Artemisia rupestris
10.6039/j.issn.1001-0408.2017.16.18
- VernacularTitle:新疆一枝蒿有效部位主要成分的制备与鉴定
- Author:
Xiaojuan RONG
;
Zhengyi GU
;
Jinhua HE
;
Lu YANG
- Keywords:
Artemisia rupestris;
LC-MS;
Preparative liquid chromatography;
Separation;
Identification;
Monomer prepara-tion;
Structure identification
- From:
China Pharmacy
2017;28(16):2227-2230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for rapid identification and efficient preparation of main ingredients in effective parts of Xinjiang Artemisia rupestris,and provide reference for researching the ethnic medicines. METHODS:LC-HRMS/MS was conducted for the preliminary study of main ingredients in effective parts of A. rupestris. HPLC,UV and MS were used to compare and analyze parts of the compounds and its reference substances,their names were determined. Column separation and preparative liquid chromatography were used for the undetermined compounds to receive monomer rapidly,and the structures were identified. RESULTS:5 compounds were separated from the effective parts,2 of which were identified as artemetin and casticin. A monomer-ic compound was obtained (yield was 0.35 mg/g,the purity was 98.5%),which was confirmed to be 6-demethoxy-4′-O-me-thoxy-capillarisin-7-O-β-D-glucoside by the structure. CONCLUSIONS:The method has achieved rapid separation,identification and preparation of target ingredients,which can be used for the fundamental research of ethnic medicine complex materials.
