Screening of theα-Glucosidase Inhibitory Active Part from Yao Medicine Pothos chinensis
10.6039/j.issn.1001-0408.2017.16.20
- VernacularTitle:瑶药葫芦钻中α-葡萄糖苷酶抑制活性部位的筛选
- Author:
Yourui XU
;
Hui YI
;
Kefeng ZHANG
;
Ying LIAO
;
Jiaxun LIN
;
Xiaoqun DUAN
- Keywords:
Yao medicine;
Pothos chinensis;
α-glucosidase inhibitor;
Different polar parts;
Mice;
Glucose tolerance test
- From:
China Pharmacy
2017;28(16):2235-2237
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To screen the α-glucosidase inhibitory active part from Pothos chinensis. METHODS:The aqueous extractions of P. chinensis were extracted by petroleum,ethyl acetate,n-butyl alcohol in turn to obtain different polarparts. Effect of each part on α-glucosidase inhibitory activity was determined,and enzyme inhibition kinetics was conducted for the screened parts with strong activity and relatively high yield rate;effects of each part on blood glucose level of mice loaded with glucose,su-crose and starch were respectively determined (using Acacoside tablet as positive control). RESULTS:Enzyme inhibition kinetics in vitro showed the ethyl acetate part [yield rate was 0.40%,enzyme activity inhibition rate was(72.90±2.85)%] had strongα-glu-cosidase inhibitory activity and showed a dose-dependent,fast,non-competitive and reversible model. Results of in vivo glucose tol-erance indicated that Acacoside tablet and each part of P. chinensis had no effects on blood glucose level of glucose-loaded mice (P>0.05);while Acacoside tablet and ethyl acetate part in P. chinensis could reduce 30,60 min blood glucose level of su-crose-loaded mice and 30,60,120 min blood glucose level of starch-loaded mice(P<0.05 or P<0.01). CONCLUSIONS:Ethyl acetate part is theα-glucosidase inhibitory active part from Yao medicine P. chinensis.
