Study on the in vitro Cell Uptake and Toxicity of Resibufogenin-loaded PLGA-TPGS Nanoparticles
10.6039/j.issn.1001-0408.2017.16.25
- VernacularTitle:脂蟾毒配基PLGA-TPGS纳米粒的体外细胞摄取及毒性研究
- Author:
Hong XU
;
Meng GAO
;
Qiuchen CHU
;
Hao DONG
;
Yu CHEN
;
Rongqian XU
;
Chenghong ZHANG
;
Yan TIAN
- Keywords:
Resibufogenin;
Lactic acid glycolic acid copolymer-water soluble vitamin E;
Nanoparticles;
Liver cancer HepG2 cells;
Ascites-type lymphatic metastasis of tumor HCa-F cells;
Cell uptake;
in vitro cytotoxicity
- From:
China Pharmacy
2017;28(16):2252-2255
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the in vitro uptake of Resibufogenin(RBG)lactic acid glycolic acid copolymer-water solu-ble vitamin E (PLGA-TPGS) in human liver cancer HepG2 cells,mouse ascites-type lymphatic metastasis of tumor HCa-F cells, and the toxicity on HepG2 cells. METHODS:RCPTN loading RBG and coumarin-6(C6)were prepared. Fluorescent inverted mi-croscope was used to observe the in vitro uptake by RCPTN HepG2,HCa-F cells. It was divided into negative control group,blank PLGA-TPGS nanoparticles(EPTN)group,5-fluorouracil solution(FS)group,RBG solution(RS)group,RBG/PLGA nanoparti-cles(RPN)group and RPTN group. WST-1 was conducted to investigate the optical density at 450 nm wavelength of HepG2 cells after 24,48,72 h incubated by FS,RS,RPN and RPTN with different final concentrations (1.25,2.5,5,10,20 μg/mL);the cell viability (CV) and half inhibitory concentration (IC50) were calculated. RESULTS:RCPTN distributed around the nucleus of HepG2,HCa-F cells. CV was decreased by RBG concentration increased in RPN group and RPTN group,and decreased by time prolonged;compared with FS group,CV in RPTN group was decreased(P<0.05 or P<0.01). IC50 of HepG2 cells incubated by FS,RS,RPN and RPTN was decreased by time prolonged,ordered by RS>FS>RPN>RPTN;IC50 incubated by RPN and RPTN for 48,72 h was obviously less than that of FS and RS(P<0.05 or P<0.01). CONCLUSIONS:RPTN can deliver RBG in-to HepG2,HCa-F cells,showing inhibition effect on HepG2 cells which is stronger than RPN,RS and FS.
