Effect of 17AAG-cypate polymer micelle on radio-sensitivityof A549 cells
10.3760/cma.j.issn.1004-4221.2017.06.015
- VernacularTitle:17AAG-cypate聚合物胶束对肺癌A549细胞放射敏感性影响
- Author:
Chenjie WU
;
Lian XUE
;
Chenglong CHEN
;
Yiru PENG
;
Huanhuan LUO
;
Dong YU
- Keywords:
17AAG-cypate micelle;
Radiosensitizing;
DNA damage repair;
Western blotting;
A549 cell
- From:
Chinese Journal of Radiation Oncology
2017;26(6):677-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the radiosensitizing effect of 17AAG-cypate micelles on human non-small cell lung cancer A549 cells and its possible mechanism.Methods (1) A single-hit multi-target model formula was used to analyze the radiosensitizing effects of 17AAG-M and 17AAG-cypate-M.(2) The effects of 17AAG-cypate-M on the viability of A549 cells under laser and X-ray irradiation were analyzed by MTT assay.(3) The effect of the drugs on the cell senescence was observed by β-galactosidase staining assay.(4) The effects of different treatment conditions on DNA damage repair were analyzed by γ-H2AX immunofluorescence staining assay.(5) The expression of p-Erk1/2 and p-Akt was measured by Western blot.The paired t test was used for analyzing the differences between groups.Results Compared with the X-ray irradiation group,the X-ray+17AAG-cypate-M group had a lower mean lethal dose and a sensitization enhancement ratio greater than 1,indicating that 17AAG-cypate-M had a radiosensitizing effect.Compared with the 17AAG-M group,the 17AAG-cypate-M group showed significantly lower cell viability (P<0.01),a significantly higher percentage of aging cells (P<0.01),and significantly further delayed DNA damage repair (P<0.01).And the 17AAG-cypate-M group had lower expression of p-Erk1/2 and p-Akt than the 17AAG-M group.Conclusions Compared with 17AAG-M,17AAG-cypate-M has a higher radiosensitizing effect on A549 cells.The mechanism might be inducing the cell senescence,delaying DNA damage repair,and inhibiting the expression of p-Erk1/2 and p-Akt.
