Down-regulating HIPK2 promotes cisplatin-induced apoptosis of human kidney tubular epithelial cells
- VernacularTitle:下调HIPK2基因表达促进顺铂诱导的肾小管上皮细胞HKC凋亡
- Author:
Jiayao LI
;
Jie WU
;
Ling QIU
- Keywords:
HIPK2;
HKC;
apoptosis;
cisplatin
- From:
Basic & Clinical Medicine
2017;37(7):1031-1036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of HIPK2 on apoptosis of human kidney tubular epithelial cells (HKC) induced by cisplatin.Methods Apoptosis of HKC cells was induced by cisplatin and the expression of HIPK2 was detected by RT-qPCR and Western blot.Two HIPK2 siRNAs were designed according to gene sequence of HIPK2 and cell lines with HIPK2 knockdown were established through transfecting the HIPK2 siRNAs into HKC cells by liposome.The expression of HIPK2 mRNA and protein was detected by RT-qPCR and Western blot after induced by cisplatin.Then cell apoptosis was detected by Annexin V/PI after the HIPK2-knockdown cells were treated with cisplatin.Moreover,the expression of pro-apoptotic protein bax was detected by Western blot after HIPK2 was knockdown.Results The expression of HIPK2 mRNA and protein was down-regulated obviously on the process of HKC apoptosis which induced by dose-dependent cisplatin (P＜0.05).The transfection of siRNA could significantly reduce the expression of HIPK2 mRNA and protein in HKC (P＜0.05),which promotes the HKC cells apoptosis induced by cisplatin.Conclusions HIPK2 can suppress the HKC cells apoptosis induced by cisplatin.
