Cytotoxic role of γδT cells to latency cells in patients with early human immunodeficiency virus-1 infection
- VernacularTitle:HIV-1早期感染者γδT细胞对储藏库细胞的细胞毒作用
- Author:
Zhen LI
;
Xiaofan LU
;
Jianping SUN
;
Bin SU
;
Hao WU
;
Yonghong ZHANG
;
Tong ZHANG
- Keywords:
human immunodeficiency virus-1;
latency;
γδ T cell;
immunotherapy
- From:
Basic & Clinical Medicine
2017;37(7):953-958
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cytotoxicity of γδ T cells to HIV-1 latency cells in patients with early HIV-1 infection.Methods Sixteen early HIV-1-infected patients were enrolled in this study.Peripheral blood mononuclear cells (PBMCs) of patients were isolated and γδ T cells were expanded using zoledronate (5 μmol/L) and interleukin (IL)-2 (1 000 IU/mL) ex vivo.Lactic dehydrogenase (LDH) was used to detect the cytotoxic role of γδ T cells to HIV-1 latency cells(J-Lat Full Length Clonel0.6).The phenotype of γδ T cells before and after expansion and the intensity of GFP in HIV-1 latency cells were detected by flow cytometry.Results Zoledronate plus IL-2 stimulated rapid and large γδ T cells proliferation ex vivo (P＜0.001).γδ T cells showed high cytotoxici ty to latency cells,and the intensity of GFP in latency cells was decreased significantly (P＜0.05).Moreover,expanded γδ T cells displayed cytotoxic NK-like phenotype,the frequency of CD56+ Vδ2 T cells in patients with early HIV-1 infection was significantly higher than that of healthy controls.Conclusions γδ T cell has an ability to eradicate HIV-1 latency,and γδ T cell-based autologous or xenogenous adoptive immunotherapy will have promise prospects to cure HIV-1 infection.
