Urotensin Ⅱ promotes collagen expression in rats cardiac tissues and proliferation of cardiac myofibroblasts in new-born rats
- VernacularTitle:尾加压素Ⅱ促进大鼠心肌胶原蛋白表达及乳鼠心肌成纤维细胞的增殖
- Author:
Wenyuan LIU
;
Qinghua HAN
;
Qinghua LIU
;
Jin WANG
- Keywords:
urotensin Ⅱ (U Ⅱ);
myocardial fibrosis;
signal transduction;
protein kinase A (PKA)
- From:
Basic & Clinical Medicine
2017;37(7):970-974
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of urotensin Ⅱ on myocardial fibrosis in rats.Methods The pressure overload animal model was established in rats by abdominal aorta coarctation.The rats were divided into sham operation group,modeled for 4,8 and 12 weeks group.The expression of U Ⅱ,GPR14,col-Ⅰ,col-Ⅲ,and PKA in cardiac tissues was detected by Western blot.Isolated and cultured cardiac myofibroblasts (CFs) from new-born SD rats were treated with U Ⅱ,KT5720 or SB-611812,and then the proliferation of CFs was observed by micro scope and CKK-8.Results The expression of U Ⅱ,GPR14,col-Ⅰ,col-Ⅲand PKA increased markedly in cardiac tissues of model rat,which were time-dependent.U Ⅱ promoted the proliferation of CFs (P＜0.05),which could be inhibited by KT5720 or SB-611812.Conclusions U Ⅱ/UT system promotes the occurring and development of myocardial fibrosis.
