miR-27a is negatively regulated by mTOR and inhibits liver cancer cell invasion via targeting GP73
- VernacularTitle:mTOR负调控miR-27a并通过靶向降低GP73抑制人肝癌细胞侵袭
- Author:
Xiaochen GAI
;
Bufu TANG
;
Fangming LIU
;
Yuting WU
;
Fang WANG
;
Hongbing ZHANG
- Keywords:
liver cancer;
mTOR;
miR-27a;
GP73;
invasion
- From:
Basic & Clinical Medicine
2017;37(7):1015-1020
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of mTOR-mediated liver cancer cell invasion.Methods q-PCR was used to check the expression of miR-27a and GP73;miR-27a mimics were transfected into GP73-high expressing M97H cells and miR-27a inhibitors were transfected into GP73-low expressing HepG2 cells,q-PCR and Western blot were performed to observe the expression of GP73;Dual-luciferase assay was also performed to verify the binding sites of miR-27a in GP73 3'UTR;miR-27a mimics were transfected into M97H cells and miR-27a inhibitors were transfected into HepG2 cells,Transwell assay was used to measure cell invasion.Results mTOR downregulated miR-27a and upregulated GP73;GP73 was downregulated by miR-27a and upregulated by miR-27a suppression;GP73 was a target gene of miR-27a;miR-27a inhibited the invasion of M97H cells rather than HepG2 cells.Conclusions miR-27a is negatively regulated by mTOR and inhibits liver cancer cell invasion via targeting GP73.
