Periostin siRNA transfection inhibits ox-LDL-induced injury in human aortic endothelial cell line
- VernacularTitle:骨膜蛋白siRNA转染抑制ox-LDL诱导的人主动脉内皮细胞系损伤
- Author:
Dong YUAN
;
Dong ZHOU
;
Anping ZHANG
;
Zhiyong LI
;
Yamin ZHANG
;
Runze SONG
- Keywords:
periostin;
endothelial injury;
cell proliferation;
cell apoptosis;
inflammatory cytokines
- From:
Basic & Clinical Medicine
2017;37(7):1026-1030
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of periostin (Postn) on oxidized low density lipoprotein (ox-LDL)-induced injury in human artery endothelial cells (HAECs) and its underlying mechanisms.Methods The HAECs were randomly divided into 4 groups:control group,ox-LDL group,Postn siRNA group and negative siRNA group.The mRNA and protein expression was analyzed by RT-qPCR and Westem blot respectively.Cell proliferation was tested by MTT.Cell apoptosis was determined by flow cytometry.NF-κB DNA binding ability was measured by EMSA.Results Compared with control group,the mRNA and protein levels of Postn were increased significantly (P＜0.05);the ability of cell proliferation was reduced (P＜0.05);the cell apoptosis rate was increased (P＜ 0.05);the protein expression levels of VCAM1,ICAM1,E-selectin,IL-1β,IL-6,TNF-α,p65 and p-IκB-α were significantly up-regulated (P＜0.05),and the NF-κB DNA binding ability was markedly increased (P＜ 0.05) in ox-LDL group,which were all reversed in Postn siRNA group.Conclusions Postn siRNA transfection may reduce ox-LDL-induced endothelial cell injury,which may be related with the inhibition of NF-κB signaling pathway.
