The relationship between expression of llotillin-1 and lymph node metastasis in early-stage cervical cancers
10.3760/cma.j.issn.1008-1372.2017.07.021
- VernacularTitle:早期宫颈癌中Flotillin-1的表达与淋巴结转移的相关性研究
- Author:
Hongling DONG
- Keywords:
Uterine cervical neoplasms;
Membrane lipids/ME;
Membrane microdomains;
Lymphatic metastasis
- From:
Journal of Chinese Physician
2017;19(7):1038-1042
- CountryChina
- Language:Chinese
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Abstract:
Objective To investigate the expression of flotillin-1 in cervical cancer,and molecular mechanism and relationship between flotillin-1 and lymph node metastasis.Methods Real-time polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry were used to detect the expression of flotillin-1 in cervical cancer cells and tissues,and detect the role of flotillin-1 in cervical cancer metastasis and the possible mechanism by over-expression and interference.Results The protein and mRNA expression of Flotillin-1 were significantly upregnlated in cervical cancer cell lines and cancer tissues.The elevated expression of flotillin-1 protein in early-stage cervical cancers was significantly associated with pelvic lymph node metastasis (P ＜0.01).Moreover,flotillin-1 up-and down-regulations remarkably affected the motility and invasion of cervical cancer cells through epithelial-mesenchymal transition (EMT) regulated by the Wnt//β-catenin and nuclear factor-κB (NF-κB) pathways.Conclusions Flotillin-1 regulates the EMT process through the catenin beta Wnt and NF-κB signaling pathway,and promotes the metastasis of cervical cancers.The flotillin-1 expression profile serves as novel independent predictor of lymph node metastasis in early-stage cervical cancers.
