Expression and identification of recombinant chimeric CARDS of Mycoplasma pneumoniae
10.3969/j.issn.1002-2694.2017.07.003
- VernacularTitle:肺炎支原体CARDS毒素蛋白多表位拼接抗原的表达及鉴定
- Author:
Baoshan LIU
;
Zhina ZHAO
;
Yujie ZHAO
;
Guizhen WANG
- Keywords:
Mycoplasma pneumonia;
CARDS toxin;
chimeric;
expression;
identification
- From:
Chinese Journal of Zoonoses
2017;33(7):588-591
- CountryChina
- Language:Chinese
-
Abstract:
We expressed multi-epitope chimeric protein of CARDS toxin protein of Mycoplasma pneumonia (Mp) in prokaryotic cells,and purified and investigated its immunoreactivity.A recombinant multi-epitope chimeric gene including ten critical epitopes was connected by linker and cloned into prokaryotic expression vector pET-28a(+),and transformed into E.coli BL21(DE3) cells for expression under induction of IPTG.The antigenicity of expressed recombinant protein was identified with 6 × His monoclonal antibody and human positive serum by Western blot.The recombinant expression vector pET-CARDS was constructed and the about 30 kDa recombinant chimeric protein expressed in BL21(DE3) successfully.Western blot analysis showed that it can react respectively with 6 × His monoclonal antibodies and human positive serum.This study showed that the chimeric CARDS protein has an obvious immunoreactivity and a potential to be a new antigen for the diagnosis of Mp infection.
