Identification and virulence analysis of a Streptococcus suis strain isolated from human blood
10.3969/j.issn.1002-2694.2017.07.005
- VernacularTitle:一株人血源猪链球菌的分离鉴定与毒力基因检测
- Author:
Yanning LYU
;
Jie LI
;
Yiwei DU
;
Xinyu LI
;
Quanyi WANG
;
Lijuan CHEN
- Keywords:
S.suis;
VITEK;
MALDI-TOF-MS;
virulence gene;
PCR
- From:
Chinese Journal of Zoonoses
2017;33(7):599-603
- CountryChina
- Language:Chinese
-
Abstract:
To identify and analyze the virulence of a bacteria strain isolated from the blood of a patient with suspected Streptococcus suis (S.suis) infection in a hospital of Beijing,we inoculated the bacteria strain isolated from the blood of the patient to the Columbia with sheep blood agar plate,after Gram staining and microscopical examination,serum agglutination test,VITEK 2 Compact microbial identification system test and matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) test,S,suis species specific gene 16SrRNA,S.suis species serotype 2 specific virulence gene capsule polysaccharide 2J (cps2J) and virulence gene muramidase-released protein (mrp),hemolysin (sly),extracellular factor protein (ef),glutamate dehydrogenase (gdh) genes,fibronectin-binding protein (fbps),glyceraldehyde-3-phosphate dehydrogenase (gapdh) genes and virulence correlated gene orf2 were further detected by PCR.Results showed that the suspicious bacteria strain of S.suis was identified as S.suis type 2 (S.suis 2) by conventional methods,MALDI-TOF-MS and PCR.PCR results showed that cps2J,sly,ef,gdh,fbps,gapdh and or f2 genes were positive,and mrp gene was negative.In conclusion,the bacteria strain isolated from the patient's blood is sly+/ef+/mrp-virulent S.suis 2.
