Highly Sensitive Detection of Spermine Based on Fluorescence Quenching of Gold Nanoclusters
10.11895/j.issn.0253-3820.160865
- VernacularTitle:金纳米簇荧光猝灭型探针高灵敏检测精胺
- Author:
Xiaogang AN
;
Jie DU
;
Weinan QI
;
Lu LIU
;
Xiaoyan LI
;
Hailing GAN
;
Xiaoquan LU
- Keywords:
Murexide;
Gold nanoclusters;
Fluorescence probe;
Spermine;
Biogenic amines
- From:
Chinese Journal of Analytical Chemistry
2017;45(8):1209-1214
- CountryChina
- Language:Chinese
-
Abstract:
A new type of fluorescent gold nanoclusters (MU-Au NCs) was prepared by hydrothermal synthesis method using ammonium benzoate murexide (MU) as reducing agent and protecting agent.The synthesis method was simple and rapid.Based on the fluorescence quenching ability of spermine, a turn off type fluorescence analysis method was established for rapid and ultra sensitive detection of spermine.The linear range for detection of spermine was 0.003-300 μmol/L and the detection limit was 1 nmol/L (S/N=3).The established analytical method of spermine provided theoretical basis and reference for construction of spermine biosensor and actual sample detection.
