Effect of ultrasound microbubble mediated miRNA delivery on the ability of migration ,invasion and clon-ing of human hepatoma HepG2 cells

VernacularTitle:超声微泡介导质粒转染对人肝癌HepG2细胞迁移、侵袭及克隆能力的影响
Author: Fangfang ZHU ; Haiqing CHEN ; Jichuang LAI ; Jialin CHEN ; Xinmin GUO
Keywords: ultrasound microbubble contrast agent; miR-21; miR-221; miR-199a; gene transfec-tion; human hepatoma HepG2 cells
From: The Journal of Practical Medicine 2017;33(16):2629-2633
CountryChina
Language:Chinese
Abstract: Objective To invstigate the effect of ultrasound microbubble mediated miRNA delivery on mi-gration,invasion and cloning ability of human hepatoma HepG2 cells. Methods The migration,invasion and col-ony forming ability of HepG2 cells were measured after transfection with antisense miRNA-21/221 and miRNA-199a mimic via the optimal ultrasound microbubble transfection method. Results Compared with the control group ,the migration ,invasion and cloning ability of cells were significantly inhibited after transfection with miRNA mimics(P < 0.05,respectively),especially for miR-199a(relative cell migration rate was 31.05%,the number of invasive cells were 38.67 ± 4.51 and the number of clones were 105.67 ± 5.86). Conclusion The pres-ent study may provide new ideas and clues for gene therapy and prognosis of hepatocell ular carcinoma through ana-lyzing the effect of miRNAs on the biological characteristics of human hepatoma HepG2 cells.