Correlation between uric acid level in the hippocampus and epilepsy in a mouse model with acute limbic seizures
10.3969/j.issn.1006-5725.2017.15.010
- VernacularTitle:边缘叶癫痫小鼠模型中癫痫危险程度与海马区尿酸水平的相关性
- Author:
Guotao YANG
;
Hongying ZHAO
;
Yin PANG
;
Aiqin DONG
- Keywords:
seizures;
kainic acid;
allopurinol;
uric acid
- From:
The Journal of Practical Medicine
2017;33(15):2464-2468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate correlation between seizures and uric acid level in the hippocampus in a mouse model with acute limbic seizures by pharmacological and genetic method. Methods Normal male C57BL/6 and mutant mice were used in this study,including urate oxidase overexpression (UOx-OE) and urate oxidase knock-out (UOx-KO) ;These mice were divided into the following groups,including the control,KA, All ,OE ,KO group respectively;during the experiment ,behaviors ,latency and duration time were recorded;dialysates were collected by microdialysis technique and uric acid level was detected by FPLC;Uric acid in the hippocampus,seizures status,latency and duration were compared. Results Twenty-four mice in total were enrolled and only 1 death occurred until the end of the study. Seizures state appeared after KA treatment. Compared to the KA group,uric acid and generalized seizures declined by the treatment of KA and All (P< 0.05) . Compared to the KA group,uric acid,latency and generalized seizures elevated in the KO group (P < 0.05). Compared to the KA group,there was no difference of latency,duration,partial and generalized seizures occurring in the OE group (P> 0.05). Conclusions Uric acid level in the hippocampus of mice may have effects on seizures ,in which it suggests that uric acid and its relevant signal pathway could be a potential therapy target in seizures clinically.
