Determination of Uric Acid Based on Active Copper-Containing Carbon Nanodots-modified Electrochemical Biosensor
10.11895/j.issn.0253-3820.170201
- VernacularTitle:基于含有活性铜的碳点电化学传感器选择性检测尿酸
- Author:
Hongying LIU
;
Tengfei JIN
;
Daxiang DAI
;
Mingru JIAO
;
Cheng HUANG
;
Mingzhen ZHANG
;
Chunchuan GU
- Keywords:
Active copper;
Carbon nanodots;
Electrochemistry;
Biosensor;
Uric acid
- From:
Chinese Journal of Analytical Chemistry
2017;45(8):1103-1108
- CountryChina
- Language:Chinese
-
Abstract:
The active copper-containing carbon nanodots were prepared by hydrothermal method, and then characterized by fluorescence spectroscopy and UV-visible absorption spectroscopy.Subsequently, a highly sensitive and selective electrochemical biosensor was fabricated on the basis of this synthesized carbon nanodots with electro-deposition technique.The electrode behavior was investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry.Furthermore, the catalysis mechanism was studied.The experimental results indicated that the biosensor exhibited a strong electrocatalytic activity toward the oxidation of uric acid (UA).What′s more, the interference from ascorbic acid and dopamine was eliminated effectively.Under the optimum conditions, there were linear relationships between the anodic peak current and the concentration of UA (1.00-300.0 μmol/L), and the limit detection was 0.30 μmol/L (S/N=3).The prepared biosensor had advantages such as easy fabrication, strong anti-interference ability, high sensitivity, and wide detection range, and could be used for real sample detection.
