Novel Tetrapeptide Hydrophilic Interaction Chromatography Materials for High-efficiency Glycopeptide Enrichment
10.11895/j.issn.0253-3820.170165
- VernacularTitle:新型四肽亲水材料用于糖肽的高效富集
- Author:
Cheng CHEN
;
Hongxi WANG
;
Hongjian KANG
;
Yao YAO
;
Guangyan QING
;
Xiuling LI
;
Xinmiao LIANG
- Keywords:
Hydrophilic interaction chromatography;
Tetrapeptide;
Glycopeptide enrichment;
Mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2017;45(8):1149-1154
- CountryChina
- Language:Chinese
-
Abstract:
In this study, a novel tetrapeptide hydrophilic interaction chromatography (HILIC) material was synthesized, and corresponding enrichment method for glycopeptides was developed.The tetrapeptide modified silica gel materials (denoted as Poly-DAPD) were synthesized by atom-transfer radical-polymerization (ATRP) and characterized by N2 adsorption desorption and thermometer, thermal gravimetric analyzer (TGA) and X-ray photoelectron spectrometer (XPS).The characterization results indicated that tetrapeptide had been successfully synthesized on silica gel.Poly-DAPD materials showed high enrichment selectivity toward fetuin glycopeptides under solid-phase extraction (SPE) mode.Comparing with commercialized ZIC-HILIC in glycopeptides enrichment of fetuin digest which mixed with 5 mole ratio of albumin bovine (BSA), the as-prepared materials showed higher selectivity in both aspects of the identified number of glycopeptides and anti-interference property.The SPE results demonstrated that the tetrapeptide-based HILIC materials could be a potential tool in large-scale glycosylation analysis.
