The role of endogenous β-glucuronidase expression in the formation of hepatolithiasis
10.3760/cma.j.issn.1007-8118.2017.07.009
- VernacularTitle:内源性β-葡萄糖醛酸酶表达在肝内胆管结石形成中的作用
- Author:
Dianbo YAO
;
Tianlin DENG
;
Changwei KE
;
Shuodong WU
- Keywords:
Hepatolithiasis;
β-glucuronidase;
C-myc;
Lipopolysaccharide (LPS)
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(7):460-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanisms involved in the regulation of endogenous β-glucuronidase expression and to explore the more effective methods to prevent recurrence of hepatolithiasis formation.Methods The expression levels of c-myc and endogenous β-glucuronidase in the liver specimens of hepatolithiasis were examined by immunohistochemical staining.The expressions of c-myc and endogenous β-glucuronidase in the human intrahepatic biliary epithelial cell line (HiBEpiC),and normal liver cell line (L02) treated with different concentrations of lipopolysaccharide (LPS) were studied using western blot.The c-myc siRNA transfection was utilized to detect the role of c-myc in the regulation of the expression of endogenous β-glucuronidase.Results Compared with normal liver samples,the expressions of endogenous β-glucuronidase and c-myc in the liver specimens of hepatolithiasis were significantly increased,and they were positively correlated with each other.LPS induced increased expressions of endogenous β-glucuronidase and c-myc in a dose-dependent manner.C-myc siRNA transfection effectively inhibited the increased expression of endogenous β-glucuronidase as induced by LPS.Conclusion LPS played a crucial role in the formation of hepatolithiasis by stimulating the endogenous expression of β-glucuronidase in liver and biliary epithelial cells via c-myc.
