Mechanism of mesenchymal stromal cells in promoting islet graft revascularization
10.3760/cma.j.issn.1007-8118.2017.07.012
- VernacularTitle:骨髓多能间质细胞促进同种异体移植胰岛新生血管形成的机制
- Author:
Rui LI
;
Hongli DONG
;
Baolin LIU
- Keywords:
Type Ⅰ diabetes mellitus;
Islet transplantation;
Mesenchymal stromal cells
- From:
Chinese Journal of Hepatobiliary Surgery
2017;23(7):474-477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanisms of mesenchymal stromal cells (MSCs) in promoting the transplanted islets neovascularization.Methods We performed co-transplantation of allogeneic islet and MSCs into non-obese diabetic (NOD) mice and the lymphocyte chimeric rate was detected by flow cytometry.The MSCs migration,transformation and secretion of cytokines were detected by immunofluorescence.Results In mice of co-combination transplantation of islet with MSCs,donor lymphocytic chimerism in graft was increased.BM-MSCs can migrate to transplanted islet,differentiate into vascular smooth muscle cells,and also secrete vascular endothelial growth factor (VEGF).Conclusions MSCs can migrate to transplanted islet and promote neovascularization.Also,it enhanced allograft immune tolerance of islet grafts via increasing donor lymphocytic chimerism.
