Lable-Free Fluorescencet Detection of Melamine Based on Its Inhibition on Synthesis of Copper Nanoclusters
10.11895/j.issn.0253-3820.170129
- VernacularTitle:基于对荧光铜纳米簇合成的抑制检测三聚氰胺
- Author:
Lijuan OU
;
Jianxin LUO
;
Aiming SUN
;
Siyu CHEN
;
Lingyun WANG
- Keywords:
Copper nanoclusters;
Cu-melamibne complex;
Fluorescence;
Label-free sensor;
Detection
- From:
Chinese Journal of Analytical Chemistry
2017;45(8):1233-1237
- CountryChina
- Language:Chinese
-
Abstract:
Based on melamine-Cu conjugate and subsequent interruption of formation of polythymine (Poly T)-templated copper nanoclusters (CuNCs), a novel fluorescent strategy was developed for detection of melamine.The method relied on the principle that the coordination of melamine to copper would be unfavorable for the reduction of Cu2+ in the poly T-CuNCs synthesis process, and thereby resulting in the decrease of fluorescence intensity of CuNCs.By this method, the fluorescence response of CuNCs displayed an linear relationship with melamine concentration in the range from 5 μmol/L to 120 μmol/L.The detection limit was 1.5 μmol/L.Furthermore, the assay was successfully applied in the detection of melamine in milk samples with good recoveries.
