Prediction of the target genes mediated by RYBP in the leukemic cell apoptosis
10.3969/j.issn.1006-5725.2017.16.004
- VernacularTitle:RYBP介导白血病细胞凋亡的靶基因预测
- Author:
Qiulan LI
;
Shunqing WANG
;
Yuping ZHANG
;
Xiaoyan CHEN
;
Jianjin XIE
;
Liangliang WU
- Keywords:
RYBP;
second-generation sequencing;
target gene
- From:
The Journal of Practical Medicine
2017;33(16):2620-2625
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the target genes in RYBP-mediated leukemia cell apoptosis by high-flux sequencing. Methods The HL-60 cell line with knockdown of RYBP was set up. MRNA and microRNA sequencing was conducted by the next-generation sequencing technology. Result MRNAs and miRNAs were dys-regulated in HL-60 cell line with knockdown of RYBP. The results of KEGG analysis indicated that the down-regu-lated genes were associated with cancer transcription regulation and amino acid metabolism. SPI1 and miR-214-3p were shown downregulated after knockdown of RYBP. Conclusion The target genes in RYBP-mediated leukemia cell apoptosis include BBC3,BAI1,SESN2 ,CCNG2,JAK3,STAT4,SPI1,BCL6,CD11b and hsa-miR-214-3p.
