Upregulation of miR-96-5p inhibits the proliferation of lung adenocarcinoma cell A549
10.3969/j.issn.1006-5725.2017.16.005
- VernacularTitle:miR-96-5p上调抑制肺腺癌细胞A549的增殖
- Author:
Degang LIU
;
Jiangzhou PENG
;
Jun LI
;
Shaoqiang ZHEN
- Keywords:
lung adenocarcinoma;
miR-96;
A549;
mTOR;
proliferation
- From:
The Journal of Practical Medicine
2017;33(16):2625-2628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hsa-miR-96 to A549. Method A549 were transfected with miR-96 inhibitor/mimics and shRNA-mTOR. The proliferation were detected by MTT,the expression of p21, cyclin D1,p-4E-BP,S6K were detected via RT-PCR and WB. The luciferase assay were used to anaylse the target of miR-96. Result With or without metformin treated,up-regulated the expression of miR-96 could inhibit A549 proliferation,increase p21 expression and decrease cyclin D1 expression instead. The results may related with G1 arrest which induced by miR-96 up-regulation. The ratio of firefly fluorescence value/Renilla fluorescence value was lower than that in wild type NC or mutant group. Conclusion miR-96 target with mTOR to inhibit the growth of A549.
