Protection effect of ERS pretreatment on the auditory cortex injury after focal cerebral ischemia-reperfu-sion injury in rats
10.3969/j.issn.1006-5725.2017.16.010
- VernacularTitle:内质网应激预处理对大鼠局灶性脑缺血再灌注后听皮层损伤的保护作用
- Author:
Zhe LV
;
Ying ZHANG
;
Yongzhou SONG
;
Meijuan SHI
;
Qing MENG
;
Hong LU
- Keywords:
endoplamic reticulum stress;
apoptosis;
ischemia-reperfusion injury;
auditory brain-stem response
- From:
The Journal of Practical Medicine
2017;33(16):2646-2649
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of endoplamic reticulum stress(ERS)pretreatment induced by 2-DG on the auditory cortex injury after focal cerebral ischemia-reperfusion injury in rats. Methods The SD rats were randomly divided into 3 groups,sham group,I/R group,and ERS pretreatment group. Tread occlusion was used to prepare the model of MCAO in the mice for 60 min followed by reperfusion for 24 h. Neurological assessment was exercised and brain infarction volume was evaluated. The auditory brainstem response was tested. The pathological changes were observed by HE staining. Neurocyte apoptosis was observed by Tunel ,and the apop-tosis index(AI)was determined. Expression of GRP78 and Caspase-12 were detected by immunohistochemistry. Results Compared with the sham group,the neurological scores,ratio of infarct volume and the hearing thresh-olds in I/R group increased significantly. HE staining showed the normal structure disappeared ,and apoptotic index increased significantly. Expression of GRP78 and Caspase-12 protein significantly up-regulated. Compared with I/R group,the indicators above showed improvement to some degrees. Conclusions The ERS pretreatment can alleviate the ischemia-reperfusion injury and neuron apoptosis in auditory cortex ,and reduce the possibility of hearing loss.
