Preparation of Graphene/Gold Nanoparticles/Glucose Oxidase Flexible Electrode Based on Poly(ethylene terephthalate) Film and Its Application in Detection of Glucose
10.11895/j.issn.0253-3820.170335
- VernacularTitle:基于聚对苯二甲酸乙二醇酯薄膜的石墨烯/金纳米粒子/葡萄糖氧化酶柔性电极的制备及检测葡萄糖
- Author:
Jie XU
;
Wen SUN
;
Luying HE
;
Qin ZHANG
;
Wenjin SU
- Keywords:
Glucose;
Soft electrode;
Glucose oxidase;
Graphene;
Gold nanoparticles
- From:
Chinese Journal of Analytical Chemistry
2017;45(8):1227-1232
- CountryChina
- Language:Chinese
-
Abstract:
The graphene prepared by chemical vapor deposition onto copper foils was transferred onto a poly(ethylene terephthalate) (PET) soft substrate by the aid of poly-methyl methacrylate (PMMA).The soft G/AuNPs/GOD composite electrode based on PET substrate was fabricated using a protocol in which the uniform distribution of Au nanoparticles (AuNPs) was firstly obtained by controlling the evaporation of gold sol on a graphene surface, then thioglycolic acid (TGA) was modified on the AuNPs through Au-S bond, and finally glucose oxidase (GOD) was immobilized on the surface of AuNPs through acylation reaction between TGA and the GOD.Glucose was detected in the linear range from 0.05 to 10.55 mmol/L with a linear correlation coefficient (r) of 0.9955.The detection was performed in phosphate buffer solution (pH 7) at 25℃ with a working potential of 0.6 V (vs.SCE electrode), and the detection limit was 1 μmol/L (3σ).The G/AuNPs/GOD flexible electrode based on PET substrate provided a new pathway to detect glucose in special environments using wearable equipment, which enlarged the applied field of glucose detection.
