Effects of overexpression of miR-30b on the biological function and tumor formation of human gastric cancer cells
10.11958/20170352
- VernacularTitle:过表达miR-30b对人胃癌细胞株SGC-7901和AGS生物学功能及其肿瘤形成的影响
- Author:
Cuicui CHEN
;
Huankun LIANG
;
Kangyan LI
;
Chengwu CHENG
;
Xipan LIU
;
Jiexing LI
;
Shuhai ZHONG
;
Licheng ZHANG
;
Laiqing LI
- Keywords:
microRNAs;
cell proliferation;
cell cycle;
apoptosis;
neoplasm invasiveness;
miR-30b
- From:
Tianjin Medical Journal
2017;45(7):677-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of overexpression of miR-30b on the proliferation,cell cycle,apoptosis and invasion of gastric cancer cell line SGC-7901 and AGS,and the inhibitory effect on the tumor formation in vivo.Methods SGC-7901 and AGS cells were transfected with miR-30b mimics and miR-control,and qRT-PCR was used to detect the expression levels of miR-30b.Western blot assay was used to detect the expression of eIFSA2 protein.CCK-8 assay was used to measure the cell proliferation.Flow cytometry was used to analyze cell cycle and apoptosis.Transwell assay was used to detect cell invasion.In addition,the SGC-7901 and AGS cells transfected with miR-30b mimics and miR-control were injected into nude mice to observe the tumor formation and the expression of eIFSA2 protein in vivo.Results Results of qRT-PCR showed that the relative expression of miR-30b was significantly higher than that of miR-control group (P ＜ 0.05).Western blot assay showed that the expression of eIF5A2 protein was decreased in miR-30b mimics group.CCK-8 assay showed that cell proliferation was inhibited in miR-30b mimics group.The result of flow cytometry showed that the cell cycle decreased and the apoptosis increased in miR-30b group.Transwell assay showed that the cell invasion was significantly lower in miR-30b group than that of control group (P ＜ 0.05).Overexpression of miR-30b inhibited the formation of tumor and decreased the expression of eIF5A2 protein in vivo.Conclusion Overexpression of miR-30b inhibits the proliferation,invasion and tumor formation of gastric cancer cells,and reduces the expression of eIF5A2 protein,which provides a potential target for gastric cancer treatment.
