An overview on application of phage display technique in immunological studies
- Author:
Rami ABBAS
;
Behdani MAHDI
;
Yardehnavi NAJMEH
;
Habibianbouhi MAHDI
;
Kazemilomedasht FATEMEH
- Keywords:
Phage display;
Antibody;
Epitope mapping;
Immunology;
Antibody library;
Peptide library
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(7):599-602
- CountryChina
- Language:Chinese
-
Abstract:
Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of mono-clonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and iso-lated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.
