Effect of NaHS on IL-1β and IL-18 secretion in adipocytes and its mechanism
10.3760/cma.j.issn.1000-6699.2017.07.012
- VernacularTitle:NaHS对脂肪细胞IL-1β、IL-18分泌的影响及其分子机制
- Author:
Tianxiao HU
;
Gang WANG
;
Qingying TAN
;
Jing WANG
- Keywords:
Obesity;
Adipocytes;
Hydrogen sulfide;
NLRP3 inflammasome
- From:
Chinese Journal of Endocrinology and Metabolism
2017;33(7):596-601
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hydrogen sulfide′s donor NaHS on the secretion of interleukin (IL)-1β and IL-18 in adipocytes and its mechanism.Methods 3T3-L1 cells were induced to differentiate into adipocytes and incubated with various concentrations of NaHS or 10 μg/ml caspase-1 inhibitor Ac-YVAD-CMK for 24 hours.The expressions of NLRP3(NLR family, pyrin domain containing 3), ASC(apoptosis associated speck-like protein containing CARD domain), caspase-1, IL-1β, and IL-18 in adipocytes, as well as the content of IL-1 and IL-18 in culture medium were determined.Results 10, 25, and 50 nmol/L NaHS significantly decreased NLRP3, ASC, and cleaved-caspase-1 protein expressions in adipocytes, as well as IL-1β and IL-18 contents in culture medium in a dose-dependent manner.The mature-IL-1β/pro-IL-1β, mature-IL-18/pro-IL-18 ratios in adipocytes and IL-1β and IL-18 contents in culture medium were also reduced by 10μg/ml Ac-YVAD-CMK.Conclusion Donor of hydrogen sulfide NaHS inhibits the maturation and secretion of IL-1β and IL-18 in adipocytes through downregulating the expression of NLRP3 inflammasome.