Early growth response gene-1 regulates host cell autophagy in HTLV-1 infection
- VernacularTitle:EGR1对HTLV-1病毒感染后宿主细胞自噬的影响
- Author:
Qingsong HUANG
;
Zhiguo NIU
;
Weidong ZHAO
;
Ziqiang DING
;
Menglin WU
;
Xiaomei HOU
;
Ruohan LYU
;
Lushuang MAO
;
Ze LI
;
Xinxiang HUANG
;
Hui WANG
- Keywords:
Early growth response gene-1;
Human T-cell leukemia virus type 1;
Autophagy;
Virus replication
- From:
Chinese Journal of Microbiology and Immunology
2017;37(7):492-496
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of early growth response gene-1 (EGR1) on the autophagy of host cells following infection with human T cell leukemia virus type 1 (HTLV-1).MethodsA HTLV-1-positive cell line MT2 was co-cultured with HeLa cells for 24 h to construct the virus early infection model.Immunoblotting assay was used to detect the expression of HTLV-1 core protein p19 and EGR1.Luciferase reporter gene analysis was used to detect the transcriptional activity of 5′-regulatory sequence of EGR1 at different time points after co-culturing.An effective small interfering RNA (siRNA) targeting EGR1 was screened out and transfected into HeLa cells by Lipofectamine 2000.Then the transfected HeLa cells were co-cultured with the HTLV-1-positive cell line MT2 for 24 h.Immunoblotting assay was used to detect HTLV-1 core protein p19, EGR1 and autophagy-related protein LC3.Real-time PCR was performed to detect viral load.Autophagosome was analyzed by immunofluorescence after co-culturing.Results The expression of EGR1 and the transcriptional activity of pEGR1-luc gradually increased after co-culturing HeLa cells with MT2 cells for 8 h (P<0.01).The expression of EGR1 was positively correlated with host cell autophagy following HTLV-1 infection.The effective siRNA for silencing the expression of EGR1 was obtained and named as siE2.The viral load, the expression of HTLV-1 core protein p19 and the proportion of LC3B/LC3A in the co-culture model were markedly down-regulated by RNA interference with siE2, which was concomitant with a persistent decrease of intracellular autophagosome (P<0.01).Conclusion EGR1 is associated with host cell autophagy and viral replication in HTLV-1 infection.
