Real-time regulatory effects of IFN-γ and programed death ligand 2 (PDL2) on adherence, proliferation and migration of human placenta-derived mesenchymal stem cells
10.3760/cma.j.issn.0254-5101.2017.07.007
- VernacularTitle:IFN-γ与PDL2对间充质干细胞黏附、增殖和迁移的实时调节作用研究
- Author:
Junzhu YI
;
Fenghuang XU
;
Feifei WANG
;
Zhuoya WANG
;
Qiang FU
;
Xiying LUAN
- Keywords:
IFN-γ;
Mesenchymal stem cell;
Adherence;
Proliferation;
Migration;
PDL2
- From:
Chinese Journal of Microbiology and Immunology
2017;37(7):514-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the real-time regulatory effects of IFN-γ, programed death ligand 2(PDL2) and janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway on the adherence, proliferation and migration of human placenta-derived mesenchymal stem cells(hPMSCs) based on a finding that IFN-γ could enhance the expression of PDL2 in hPMSCs through JAK/STAT signaling pathway.Methods hPMSCs were isolated by using enzyme digestion method and then co-cultured with IFN-γ, anti-PDL2 monoclonal antibody (anti-PDL2 McAb) and JAK inhibitor, respectively.Real-time cell analysis (RTCA) was used to detect the dynamic changes in the adherence, proliferation and migration of hPMSCs following various interventions.Results IFN-γ remarkably suppressed hPMSCs proliferation during the period from 40 hours to 80 hours after intervention and also inhibited the non-targeted migration of hPMSCs.However, hPMSCs adherence was not affected by IFN-γ.Co-culturing hPMSCs with anti-PDL2 McAb significantly enhanced hPMSCs adhesion and inhibited their non-targeted migration, but had no significant effect on hPMSCs proliferation.Furthermore, the proliferation of hPMSCs co-cultured with IFN-γ and anti-PDL2 McAb was significantly inhibited as compared with that of anti-PDL2McAb treatment group.The adhesion, migration and proliferation of hPMSCs were significantly inhibited after co-culturing them with JAK inhibitor.Conclusion IFN-γ can remarkably suppress the proliferation and migration of hPMSCs.PDL2 can enhance the migration and inhibit the adhesion of hPMSCs.JAK/STAT signaling pathway is involved in regulating the adhesion, migration and proliferation of hPMSCs.
