Determination of Glycohyodeoxycholic Acid and Glycochenodeoxycholic Acid in Pulvis Fellis Suis by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定猪胆粉中的甘氨猪去氧胆酸和甘氨鹅去氧胆酸的含量
- Author:
Limin WANG
- Keywords:
Pulvis fellis suis;
Glycohyodeoxycholic acid;
Glycochenodeoxycholic acid;
HPLC-ELSD
- From:
China Pharmacist
2017;20(8):1480-1482
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the simultaneous determination of glycohyodeoxycholic acid and glycochenodeoxycholic acid in pulvis fellis suis by HPLC-ELSD.Methods: The analysis of methanol extract of pulvis fellis suis was performed on a Welch Ultimate Plus C 18 (250 mm× 4.6 mm ,5 μm)column, and the mobile phase consisted of acetonitrile and 0.1% formic acid (40∶60).The flow rate was 1.0 ml ·min-1.Alltech ELSD 2000ES was used as the detector, the drift tube temperature was 105℃ and the nitrogen flow rate was 2.5 L·min-1.Results: Glycohyodeoxycholic acid and glycochenodeoxycholic acid had good linear relationships within the range of 0.306-4.590 μg (r=0.999 5) and 0.280-4.194 μg (r=0.999 4), respectively.The average recovery was 99.23% with RSD of 2.0% and 100.37% with RSD of 1.8% (n =9), respectively.Conclusion: The method is accurate, sensitive and repeatable, which can be used for the quality control of pulvis fellis suis.
