Determination and analysis of free amino acids in snake venom
10.3969/j.issn.1005-1678.2017.08.006
- VernacularTitle:矛头蝮蛇蛇毒中游离氨基酸测定与分析
- Author:
Yan XUE
;
Xiulin LI
;
Xiuna LI
;
Zhongfu DING
;
Liangliang CUI
- Keywords:
snake venom;
amino acids;
phenyl isothiocyanate;
HPLC
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(8):14-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for simulataneous determination of 18 free amino acids in snake venom. Methods Preparation of free amino acid samples by membrane. HPLC analysis was performed after derivatization by using phenyl isothiocyanate (PITC) as a derivative reagent, samples were analyzed on Ultimate LP-C18 column with gradient elution column of 0.05 mol/L sodium acetate buffer and methanol-acetonitrile- water (40:40:20), and current speed was1.0 mL/min, and the column temperature was set at 35℃, and detection wavelength was 254nm. Results The 18 kinds of amino acids showed a good linearity with the correlation coefficients ≥0.99. The recovery rate was 74.59%~110.62%. Snake venom contained 17 kinds of amino acids, the total content of amino acids was 0.2%. Conclusion The method was accurate, reproducible and reliable, and can be used for the determination of amino acids in snake venom and related products.
