Study on Enrichment Process of Total Flavonoids from Crataegus pinnatifida Bge, with Macroporous Resin
10.3969/j.issn.1005-1678.2017.08.003
- VernacularTitle:大孔树脂吸附法富集山楂叶总黄酮的工艺研究
- Author:
Yuhua ZHAO
;
Mingzheng CEN
;
Haizhu FAN
- Keywords:
Crataegus pinnatifida Bge;
total flavonoids;
macroporous resin;
enrichment;
orthogonal experiment
- From:
Chinese Journal of Biochemical Pharmaceutics
2017;37(8):7-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the optimal technology parameters of the enrichment process for total flavonoids of Crataegus pinnatifida Bge,with macroporous Resin. Methods The enrichment process was studied by orthogonal experiment design using orthogonal from L9(34) with the content of total flavonoids from Crataegus pinnatifida Bge,as indexes, the factors such as adsorption capacity, the column diameter-height ratio, eluant volume and adsorption time were investigated. Results The optimum elution parameters were as follows: the eluant volume of 60% alcohol—the wet resin amount (g)—the sample volume (mL) was 2:1, the sample was 4:1, the column diameter-height ratio was 1:6 , the adsorption time was 1h, respectively. Conclusion This process enriching the total flavonoids of Crataegus pinnatifida Bge,is feasible.
