Cytotoxicity and mechanism of zinc oxide nanoparticles on murine macrophage Ana-1 cells
10.3867/j.issn.1000-3002.2017.06.020
- VernacularTitle:纳米氧化锌对小鼠腹腔巨噬细胞Ana-1的毒性作用及机制
- Author:
Tianxue CHU
;
Liguo XING
;
Juan YUAN
;
Wei DAI
;
Yuan WANG
;
Xinyu LONG
;
Ting LIU
;
Daiying ZUO
;
Yingliang WU
- Keywords:
nanoparticles;
zinc oxide;
Ana-1 cells;
cytotoxicity
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(6):636-641
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the toxicity of zinc oxide nanoparticles (ZnO-NPs) on murine macrophage Ana-1 cells and the mechanism.METHODS Ana-1 cells were incubated with ZnO-NP (2.5-160 mg· L-1).Cell viability was investigated by MTT assay.The integrity of cell membrane was investigated by acridine orange-ethidium bromide (AO-EB) staining.The intracellular uptake of ZnO-NP and the percentage of sub-G1 of Ana-1 cells were detected by flow cytometry.Zinc ions were determined by fluorescent probe.The change of cell viability was studied after chelating zinc ions with ethylene diamine tetraacetic acid (EDTA).RESULTS ZnO-NP 2.5,5,10 and 20 mg· L-1 decreased cell viability of Ana-1 cells (r=0.905,P＜0.05) in a concentration-dependent manner.The cell viability was decreased to 27.9％ after exposure to ZnO-NP 20 mg· L-1.Intracellular uptake of ZnO-NP was increased after Ana-1 cell incubated with ZnO-NP at concentrations ranging from 40 to 160 mg· L-1 (P＜0.05).There were obvious free zinc ions in the cells.EDTA 2.5 mmol· L-1 significantly increased the cell viability decreased by ZnO-NP 20 mg· L-1 (P＜0.05).Chelating free zinc ions significantly mitigated ZnO-NP induced cell toxicity (P＜0.05).CONCLUSION Cytotoxicity and apoptosis of Ana-1 cells induced by ZnO-NP might be related to intracellular uptake of ZnO-NP and release of zinc ions.
